This report provides exploratory and preliminary correlation analyses between sex hormones and T2 final hippocampal subfield volume data from the Healthy Aging Study sample that have completed the spatial navigation study.
Cleaning data steps included (T2 subfields):
Filter participants that have useable T2 subfield data from scan and completed spatial navigation behavior
Select sex hormone measures from when spatial study was conducted
Values were already averaged together (left and right) and already corrected with TIV
Calculating Total Hippocampal Volume:
Calculating Total MTL:
| Sex | n | Mean Age | SD Age | n | Mean Age | SD Age | n | Mean Age | SD Age | n | Mean Age | SD Age |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Female | 55 | 50.49 | 3.37 | 15 | 50.27 | 3.26 | 45 | 50.07 | 3.17 | 32 | 50.28 | 3.05 |
| Male | 22 | 49.95 | 3.39 | 11 | 49.18 | 2.93 | 14 | 49.71 | 3.52 | 19 | 49.74 | 3.33 |
Above table shows the table of sample size and mean and standard deviation of age by sex for overall group who have completed spatial behavior and have a useable T2hipp scan.
For women, 60 had T2 hipp scan completed and completed an aspect of spatial navigation task but 5 were removed due to poor quality scans. For men, 25 had T2 hipp scan completed and completed an aspect of spatial navigation task but 3 were removed for poor quality scans. Thus n = 55 for women and n = 22 for men.
All sample size for T2hipp x hormone correlations for women are listed in the table above. For men, because some participants did not have all hormones evaluated (e.g, only testosterone available), the sample size for the correlations for that hormone and with T1 hipp volume will be different.
For men, the accurate sample sizes would be:
LOOP: n = 10 DHEAS, n = 11 for SHBG and T
MAZE: n = 13 DHEAS, n = 14 for SHBG and T
DSP: n = 18 DHEAS, n = 19 for SHBG and T
Structure: Shapiro-Wilk’s test reveal that total hipp and MTL volumes were normally distributed.
Subfields: Shapiro-Wilk’s test reveal that CA1 for overall and women, and PHC for women volumes were not normally distributed. Hormone and subfield/volume associations for these subfields will be using Spearman rank correlations.
Shapiro-Wilk tests reveal that all six hormones violated normality in the overall and women group. Most hormones for women subgroup distributions were still significantly different from normal after log transformation, so Spearman rank correlations will be applied to these hormone and subfield and structure associations. For men, only SHBG violated normality so all subfield and structure correlations with this hormone in men will be spearman rank.
Shapiro-Wilk’s test reveal that MAZE accuracy for all and MAZE moves for overall, women groups were not normally distributed. DSP solution index for overall and women were also not normally distributed. Behavior and volume correlations will be Spearman rank
| Reproductive Status | n | Mean Total Hipp Volume | SD Total Hipp Volume |
|---|---|---|---|
| Pre | 22 | 1.18 | 0.14 |
| Peri | 18 | 1.16 | 0.13 |
| Post | 15 | 1.17 | 0.14 |
| Sex | n | Mean Total Hipp Volume | SD Total Hipp Volume |
|---|---|---|---|
| Female | 55 | 1.17 | 0.14 |
| Male | 22 | 1.16 | 0.17 |
Means and standard deviations of total hipp are displayed by reproductive status and by sex.
ANOVA results comparing T2 hipp GMV by reproductive status was not significant.
T-test results comparing GMV by sex was not significant.
All correlations are Spearman.
All correlations are Pearson except for SHBG (Spearman)
All correlations are Pearson
All correlations are Pearson
All correlations are Spearman.
All Spearman correlations except for men (Pearson).
All correlations were Pearson
All correlations were Spearman except for men (Pearson).
| Reproductive Status | n | Mean MTL Volume | SD MTL Volume |
|---|---|---|---|
| Pre | 22 | 4.97 | 0.50 |
| Peri | 18 | 4.73 | 0.54 |
| Post | 15 | 4.90 | 0.54 |
| Sex | n | Mean MTL Volume | SD MTL Volume |
|---|---|---|---|
| Female | 55 | 4.87 | 0.53 |
| Male | 22 | 4.68 | 0.62 |
Means and standard deviations of MTL are displayed by reproductive status and by sex.
ANOVA results comparing MTL by reproductive status was not significant.
T-test results comparing MTL by sex was not significant.
All correlations are Spearman.
All correlations are Pearson except SHBG (Spearman)
All correlations are Pearson
All correlations are Pearson
All correlations are Spearman
All correlations are Spearman except for men (Pearson)
All correlations are Pearson
All correlations were Spearman except for men (Pearson)
| Reproductive Status | n | Mean CA1 Volume | SD CA1 Volume |
|---|---|---|---|
| Pre | 22 | 0.43 | 0.05 |
| Peri | 18 | 0.43 | 0.05 |
| Post | 15 | 0.43 | 0.05 |
| Sex | n | Mean CA1 Volume | SD CA1 Volume |
|---|---|---|---|
| Female | 55 | 0.43 | 0.05 |
| Male | 22 | 0.42 | 0.06 |
Means and standard deviations of the subfield are displayed by reproductive status and by sex
ANOVA results comparing CA1 subfield by reproductive status was not significant.
T-test results comparing CA1 subfield by sex was not significant.
All correlations are Spearman.
Correlations are all Pearson except SHBG (Spearman)
All correlations were Spearman except for men (Pearson)
All correlations were Spearman except for men (Pearson)
All correlations were Spearman
All correlations were Spearman except for men (Pearson)
All correlations were Spearman except for men (Pearson)
All correlations were Spearman except for men (Pearson)
| Reproductive Status | n | Mean CA23 Volume | SD CA23 Volume |
|---|---|---|---|
| Pre | 22 | 0.15 | 0.03 |
| Peri | 18 | 0.14 | 0.02 |
| Post | 15 | 0.15 | 0.02 |
| Sex | n | Mean CA23 Volume | SD CA23 Volume |
|---|---|---|---|
| Female | 55 | 0.14 | 0.02 |
| Male | 22 | 0.14 | 0.02 |
Means and standard deviations of the subfield are displayed by reproductive status and by sex
ANOVA results comparing CA23 subfield by reproductive status was not significant.
T-test results comparing CA23 subfield by sex was not significant.
All correlations were Spearman
All correlations are Pearson except SHBG (Spearman)
All correlations are Pearson
All correlations are Pearson
All correlations are Pearson
All correlations are Spearman except for men (Pearson)
All correlations are Pearson
All correlations are Spearman except for men (Pearson)
| Reproductive Status | n | Mean DG Volume | SD DG Volume |
|---|---|---|---|
| Pre | 22 | 0.37 | 0.05 |
| Peri | 18 | 0.36 | 0.05 |
| Post | 15 | 0.37 | 0.06 |
| Sex | n | Mean DG Volume | SD DG Volume |
|---|---|---|---|
| Female | 55 | 0.37 | 0.05 |
| Male | 22 | 0.38 | 0.08 |
Means and standard deviations of the subfield are displayed by reproductive status and by sex
ANOVA results comparing DG subfield by reproductive status was not significant.
T-test results comparing DG subfield by sex was not significant.
All correlations are Spearman
All correlations are Pearson except SHBG (Spearman)
All correlations were Pearson
All correlations were Pearson
All correlations were Spearman
All correlations were Spearman except for men (Pearson)
All correlations were Pearson
All correlations were Spearman except for men (Pearson)
| Reproductive Status | n | Mean ERC Volume | SD ERC Volume |
|---|---|---|---|
| Pre | 22 | 0.71 | 0.19 |
| Peri | 18 | 0.65 | 0.15 |
| Post | 15 | 0.66 | 0.11 |
| Sex | n | Mean ERC Volume | SD ERC Volume |
|---|---|---|---|
| Female | 55 | 0.68 | 0.16 |
| Male | 22 | 0.60 | 0.12 |
Means and standard deviations of the subfield are displayed by reproductive status and by sex
ANOVA results comparing ERC subfield by reproductive status was not significant.
T-test results comparing ERC subfield by sex was significant. Women (M = 0.678) have larger ERC volume than men (M = 0.601) (t = 2.393, df = 52.818, p = 0.02).
All correlations are Spearman.
All correlations are Pearson except SHBG (Spearman)
All correlations were Pearson
All correlations were Pearson
All correlations were Spearman
All correlations were Spearman except for men (Pearson)
All correlations are Pearson
All correlations were Spearman except for men (Pearson)
| Reproductive Status | n | Mean PHC Volume | SD PHC Volume |
|---|---|---|---|
| Pre | 22 | 1.61 | 0.23 |
| Peri | 18 | 1.56 | 0.16 |
| Post | 15 | 1.57 | 0.22 |
| Sex | n | Mean PHC Volume | SD PHC Volume |
|---|---|---|---|
| Female | 55 | 1.58 | 0.21 |
| Male | 22 | 1.50 | 0.24 |
Means and standard deviations of the subfield are displayed by reproductive status and by sex
ANOVA results comparing PHC subfield by reproductive status was not significant.
T-test results comparing PHC subfield by sex was not significant.
All correlations are Spearman
All correlations are Pearson except SHBG (Spearman)
Overall and male correlations were Pearson, female and repo groups were Spearman.
Overall and male correlations were Pearson, female and repo groups were Spearman.
All correlations were Spearman
All correlations were Spearman except for men (Pearson)
Overall and male correlations were Pearson, female and repo groups were Spearman.
All correlations were Spearman except for men (Pearson)
| Reproductive Status | n | Mean PRC Volume | SD PRC Volume |
|---|---|---|---|
| Pre | 22 | 1.47 | 0.26 |
| Peri | 18 | 1.37 | 0.28 |
| Post | 15 | 1.49 | 0.26 |
| Sex | n | Mean PRC Volume | SD PRC Volume |
|---|---|---|---|
| Female | 55 | 1.44 | 0.27 |
| Male | 22 | 1.42 | 0.38 |
Means and standard deviations of the subfield are displayed by reproductive status and by sex
ANOVA results comparing PRC subfield by reproductive status was not significant.
T-test results comparing PRC subfield by sex was not significant.
All correlations are Spearman
All correlations are Pearson except SHBG (Spearman)
All correlations were Pearson
All correlations were Pearson
All correlations were Spearman
All correlations were Spearman except for men (Pearson)
All correlations were Pearson
All correlations were Spearman except for men (Pearson)
| Reproductive Status | n | Mean SUB Volume | SD SUB Volume |
|---|---|---|---|
| Pre | 22 | 0.23 | 0.03 |
| Peri | 18 | 0.23 | 0.03 |
| Post | 15 | 0.23 | 0.04 |
| Sex | n | Mean SUB Volume | SD SUB Volume |
|---|---|---|---|
| Female | 55 | 0.23 | 0.04 |
| Male | 22 | 0.22 | 0.03 |
Means and standard deviations of the subfield are displayed by reproductive status and by sex
ANOVA results comparing SUB subfield by reproductive status was not significant.
T-test results comparing SUB subfield by sex was not significant.
All correlations are Spearman.
All correlations are Pearson except SHBG (Spearman)
All correlations were Pearson
All correlations were Pearson
All correlations were Spearman
All correlations were Spearman except for men (Pearson)
All correlations were Pearson
All correlations were Spearman except for men (Pearson)
Sample included HAS participants who had a T2 hipp scan and completed spatial navigation behavior.
As of 12/07/2022, it’s likely that there are 2 more subjects to be added to analysis (subjects male 412 and pre female 425) since their MRI scans were missing.
All hormones were skewed for overall and women groups, so hormone and volume correlations were Spearman rank correlations. All correlations for men were Pearson except for those involving SHBG.
Total hipp Volume and MTL
Total hipp volume and MTL volumes were normally distributed. ANOVA results comparing total hipp volume and MTL by reproductive stage were not significant. T-test results comparing total hipp volume and MTL by sex were also not significant.
For total hipp volume, higher progesterone concentration was negatively associated with lower total hipp volumes in women (p < 0.05, Spearman) and in pres (p < 0.001, Spearman). Higher DHEAS was significantly negatively associated with total hipp volume for posts (p < 0.05, Spearman). Higher FSH was marginally positively associated with total hipp volume for peris (p = 0.069, Spearman).
For MTL, progesterone was marginally negatively associated with lower MTL volumes in women (p = 0.051, Spearman). Higher testosterone was marginally positively associated with MTL volume for pres (p = 0.069, Spearman).
Subfields
Subregion volumes for women and men were generally normally distributed, except for CA1 overall and in women, and PHC in women. ANOVA results comparing subfields by reproductive stage were all not significant. T-test results comparing subfields by sex were all not significant except for ERC (p < 0.05), with women showing greater ERC subfield volume than men.
For estradiol, it was significantly negatively correlated with PRC for posts (p < 0.05, Spearman). It was also marginally negatively associated with PHC for pres (p = 0.062, Spearman).
For progesterone, it was significantly negatively associated with SUB for pres (p < 0.05, Spearman), with PRC for posts (p < 0.05, Spearman), DG for women (p < 0.01, Spearman) and DG for pres (p < 0.01, Spearman), as well as with CA1 for pres (p < 0.05, Spearman). Progesterone was only marginally positively associated with CA1 for posts (p = 0.052, Spearman).
For FSH, it was significantly negatively correlated with CA1 for posts (p < 0.05, Spearman). It was significantly positively correlated with ERC for peris (p < 0.05, Spearman) and SUB for pres (p < 0.05, Spearman). FSH was marginally positively correlated with CA23 for peris (p = 0.058, Spearman) and with DG for peris (p = 0.069, Spearman) and with DG for peris (p = 0.069, Spearman).
For SHBG, it was significantly negatively correlated with PHC for posts (p < 0.05, Spearman), and marginally negatively correlated with SUB for pres (p = 0.068, Spearman).
For DHEAS, it was significantly negatively correlated with CA23 for posts (p < 0.01, Spearman) and with DG for posts (p < 0.05, Spearman).
For testosterone, it was significantly positively associated with PHC for peris (p < 0.05, Spearman). Testosterone was also marginally negatively correlated with CA1 for men (p = 0.066, Pearson).
Total hipp Volume and MTL
LOOP greater degrees traveled was significantly negatively correlated with lower total hipp volume within overall (women and men together) (p < 0.01, Pearson) and within men (p < 0.05, Pearson).
DSP higher solution index was marginally negatively correlated with lower total hipp volume in men (p = 0.052, Pearson).
There were no significant or marginal correlations observed for MTL and navigation behaviors.
Subfields
LOOP larger position error was significantly negatively correlated with lower PHC volume within overall (women and men) (p < 0.05, Pearson).
LOOP greater degrees traveled was significantly negatively correlated with lower volumes in CA1 for overall (p < 0.05, Spearman) and for men (p = 0.05, Pearson), in CA23 for overall (p < 0.01, Pearson) and for men (p < 0.05, Pearson), in DG for overall (p < 0.05, Pearson) and for men (p < 0.05, Pearson), in ERC for peris (p < 0.05, Pearson), in PRC for peris (p < 0.05, Pearson), and in SUB for overall (p < 0.05, Pearson). Basically pattern can be seen in all subfields except for PHC.
There were no significant or marginal correlations observed for MAZE accuracy.
MAZE higher moves made was significantly positively associated with higher PHC volume in pres (p < 0.05, Spearman).
DSP higher wayfinding success was significantly negatively correlated lower ERC volumes in pres (p < 0.05, Pearson), but marginally with overall (p = 0.05, Spearman). It was significantly positively associated with PHC for persi (p < 0.05, Spearman) but only marginally in women (p = 0.05, Spearman).
DSP higher solution index was significantly positively correlated with higher volume in CA23 in peris (p < 0.05, Spearman). It was marginally negatively correlated with lower volume in DG for overall (p = 0.069, Spearman) and for men (p = 0.05, Pearson), and also in SUB for men (p = 0.063, Pear).